Human CRISPRi sgRNA Library (UbiC-RFP-Puro) (DNA)-academia


Quantity: 200 ug

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    • Genome-Wide Coverage with a Single 106K Module
    • Single library with ~106,000 constructs
    • The promoter region of each gene is targeted by 5 sgRNAs
    • All human protein-coding genes covered—approximately 19,000 gene targets

    Genome-wide pooled library of ~106,000 sgRNA constructs cloned in the pRSG16-U6-sg-UbiC-TagRFP-2A-Puro vector targeting the promoter region of ~19,000 protein-coding human genes. Engineered to work with deactivated spCas9 (dCas9) complexed with the KRAB repressor to inactivate or down regulate gene expression.
    Each gene is targeted by 5 sgRNAs, specially designed according to Horbeck, et al., eLife 2016;5:e19760 DOI: 10.7554.

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    Storage Temperature: -20C