NGS DNA Fragmentation and Library Prep Kit (Ion Torrent), with indexed adaptors


Quantity: 48 rxns

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     Fast and convenient High Quality NGS DNA Fragmentation and Library Prep for 100 ng – 500 ng genomic DNA inputs

    • Fast: 1-hour library construction from intact genomic DNA to NGS library, Hands-on time: 5 min
    • Intact genomic DNA can be used directly as input; prior DNA fragmentation is not required.
    • Works with both EDTA-free DNA samples and DNA samples in TE buffer
    • Simple workflow: Three steps in one tube; only one beads purification step
    • Guaranteed high library conversion efficiency
    • Input DNA amount: from 100 ng to 500 ng genomic DNA

    The NGS DNA Fragmentation & Library Prep Kit (ion torrent platform) was developed for construction of high quality libraries for next generation sequencing on the Ion Torrent platform. The kit uses intact genomic DNA (both EDTA-free DNA and DNA in TE buffer are compatible) as input DNA without prior DNA fragmentation step. BioDynami´s NGS kit has a fast and simple workflow with only three steps, all performed in 1 tube. The DNA libraries can be generated within one hour with less than 10 minutes of hands-on time. Library multiplexing up to 12 samples is possible.

    The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of prior mechanical DNA shearing or enzymatic DNA fragmentation. The protocol is optimized to generate libraries from 200 bp to 500 bp in size. The library size is inversely correlated with the incubation time of step 1 at 20°C. BioDynami´s unique library prep technology increases library conversion efficiency and eliminates insert concatemer ligation. The kit is available without indexing (Cat.# 30052) and for library multiplexing of up to 12 samples (Cat.# 30053)

    Shipping Conditions: Dry Ice
    Storage Temperature: -20C