Description
Generate WGS libraries for any genome size from 50 Mb to 5 Gb or prepare Metagenomics libraries
• 288 paired end TELL-Seq WGS libraries from samples with small genomes (50 Mb to 200 Mb) or
• 144 paired end TELL-Seq WGS libraries from samples with medium sized genomes (200 Mb to 1 Gb) or
• 96 paired end TELL-Seq WGS libraries from samples with large sized genomes (1 Gb to 5 Gb)
• 96 paired end TELL-Seq Metagenomics libraries from microbiome samples (2.5 – 5 ng HMW genomic DNA)
This complete bundle kit includes the following components:
– 4 x Cat.# 110004: 4 x Eukaryotic Genomes and Metagenomics Library Reagent Box 1, HT24 (TELL-Seq enzymes and incubation buffers, storage at
minus 20 C) and 4 x Eukaryotic Genomes and Metagenomics Library Reagent Box 2, HT24 (TELL-Seq barcoding beads, storage at plus 4 C).
– Cat.# 100043: TELL-Seq Library Multiplex Primer Set for High Throughput (C1 – C96, plated) (minus 20 C)
– Cat.# 100013: TELL-Seq Illumina Sequencing Primers, HT (minus 20 C)
All components can also be ordered individually.
TELL-Seq: Long-Read Sequencing for All
• Up to 200 kb sequence length on Illumina by barcode linked reads
• Ultralow DNA Input: 0.5 to 5 ng HMW gDNA depending on genome size
• Simple single tube protocol – sequencing ready library in 3 hours
• Easy to automate for high throughput applications
• Free TELL-Seq software packages available for performing data analysis yourself
The TELL-Seq WGS library prep kit uses the highly innovative Transposase Enzyme Linked Long-read Sequencing (TELL-Seq™) technology ( Chen et al.,Genome Research, June 2020 ) for preparing a paired-end library to generate barcode linked highly accurate reads from an Illumina® sequencing system. Coupled with free TELL-Seq analysis software, the linked reads can be easily used for the following applications and more:
• de novo genome assembly
• Whole genome haplotype phasing
• Genome wide structural variant calling
- High resolution Metagenomics-Assembled Genomes (MAGs)
- Highly accurate species identification & abundance quantification
- Discovery of new microbial species & strains and their genomes