ExoGAG Exosome Purification Kit

Description

ExoGAG:  The Next Generation of Exosome Isolation developed for Biomarker Detection in Liquid Biopsy

Exosome purification for accurate downstream analysis of exosomal proteins and nucleic acids

ExoGAG is a patented extracellular vesicles purification method developed by NEXOTECH (Lugo, Spain) for isolation of EVs from liquid biopsy samples – free of non-EV contaminations.  It enables the accurate analysis of proteins or genetic material contained in the EVs, which is ideal for genuine biomarker detection.

ExoGAG uses a simple precipitation protocol to specifically isolate exosomes and other extracellular vesicles. The ExoGAG technology is based on glycosaminoglycans (GAGs) coating exosomes and other EVs. The negatively charged GAGs are neutralized by the positive charge of cationic agents in the ExoGAG solution leading to a complex that can be easily purified by centrifugation (Herrero et al, Cancers 2019).

• For small liquid biopsy inputs (plasma, serum, urine etc.)
• Easy, fast, and scalable precipitation protocol using a standard lab centrifuge
• Isolates pure exosomes/EVs at high yields
• Ideal for true biomarker detection and screening

After a short incubation with the liquid biopsy sample such as plasma, serum, urine, etc., pure exosomes are isolated by a brief standard centrifugation, whereas non-exosomal protein, DNA, & RNA contaminations are removed. The purified exosomes can be detected by NTA, and can be used to analyze exosome-specific proteins, DNA, mRNA, miRNA and other small RNAs at highest accuracy.

Citations:

Herrero et al, Cancers 2019: Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer

Mercadal et al., Journal of Molecular Sciences 2020: Impact of Extracellular Vesicle Isolation Methods on Downstream miRNA Analysis in Semen: A Comparative Study

Herrero et al, Biomedicines 2023: Extracellular Vesicles’ Genetic Cargo as Noninvasive Biomarkers in Cancer: A Pilot Study Using ExoGAG Technology

ExoGAG-purified Extracellular Vesicles can be used for the following downstream applications:

• NTA
• FACS
• ELISA
• (Glyco-)Protein Microarrays
• qRT-PCR for miRNA/mRNA detection
• BEAMing and Digital PCR for allelic variant and methylation analysis
• DNA-and RNA-seq including Small RNA-seq